Wrky proteiner: signalering och reglering av uttryck under abiotiska

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In the ABA-ABAR-WRKY40-ABI5 core signaling complex, WRKY40 acts to suppress ABA signaling, and high levels of ABA promote ABAR-WRKY40 interactions, then activate expression of ABA responsive genes, including the ABA responsive transcription factors ABI5 and ABI4. J. Exp. Bot., 2017. Arabidopsis ABI5 plays a role in regulating ROS homeostasis by activating CATALASE 1 transcription in seed germination Plant Molecular Biology , Apr 2017 Chao Bi , Yu Ma , Zhen Wu , Yong-Tao Yu , Shan Liang , Kai Lu , Xiao-Fang Wang Additionally, NF-YC9 up-regulates expression of the ABI5 gene in response to ABA. These findings show that NF-YC9 may be involved in ABA signaling as a positive regulator and likely functions redundantly together with other NF-YC members, and support the model that the NF-YC9 mediates ABA signaling via targeting to and aiding the ABA-responsive transcription factors such as ABI5. ABI5 affects reactive oxygen species (ROS) homeostasis by affecting CATALASE expression and catalase activity. Furthermore, we showed that ABI5 directly binds to the CAT1 promoter and activates CAT1 expression.

Abi5 overexpression

Results In vitro kinase assay results showed that Thr-128, Ser-134, and Thr-451 of ABF3 are calcium 2020-12-01 · FOF2 inhibits ABA-mediated seed germination partially by repressing ABI3 and ABI5. • Overexpression of FOF2 enhances plant drought tolerance. • FOF2 promotes ABA-induced stomatal closure and ABA biosynthesis under drought stress. Direct targets of ABI4 and ABI5 regulation. To emphasize the effects of ABI4 and ABI5 expression, we analyzed 11 day old seedlings, a developmental stage when seed specific transcription factors are not normally expressed but overexpression of ABI4 or ABI5 enhances ABA-inducible responses (Brocard et al. 2002; Söderman et al.

Taken together, our results demonstrate that PPC2 and ABI5 are key regulators of plant acclimation to low CO 2, and positively contribute to carbon fixation and metabolism in C 3 plants. ABI5 was previously reported as the target protein of AFP2 during seed germination (Lopez-Molina et al., 2003), and overexpression of ABI5 activates the flowering negative factor FLC at the transcriptional level to repress flowering (Wang et al., 2013b).

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By contrast, ABI5 regulates the expression of PYL11 and PYL12 by directly binding to their promoters. Moreover, the expression of eight other PYLs is … Loss‐of‐function in ABI5 (abi5) promotes juvenile‐to‐adult transition, whereas overexpression of ABI5 delays this transition in short days.

Wrky proteiner: signalering och reglering av uttryck under abiotiska

In the present experiment, we showed that 3-amino-1,2,4-triazole (3-AT) inhibits seed germination of the loss-of-function mutant abi5-1, but promotes seed germination of the ABI5-overexpression transgenic line, and that ABI5 affects reactive oxygen species (ROS) homeostasis. ABI5 overexpression rescued the growth arrest phenotype at low CO 2. Taken together, our results demonstrate that PPC2 and ABI5 are key regulators of plant acclimation to low CO 2, and positively contribute to carbon fixation and metabolism in C 3 plants. Pharmacological assay showed that abi5-1 mutant was insensitive to TOR inhibitor AZD8055, whereas AtABI5 overexpression lines were hypersensitive to AZD8055 in Arabidopsis. Biochemical interaction assays demonstrated that ABI5 physically interacted with the RIBOSOMAL S6 KINASE2 (S6K2) protein in plant cell.

ABI5 is mainly expressed in dry seeds, and its expression markedly decreases after germination (Finkelstein and Lynch, 2000b; Lopez‐Molina et al., 2001). Expression of ABI5 is strongly induced by exogenous ABA (Lopez‐Molina et al., 2001). ABI5 in the hy5 mutant background restored its ABA sensitivity and overexpression of ABI5 in the wild-type background-enhanced light response.
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The expression level of ACTIN2 represents the loading control. of the ABI5 and its target genes were greatly reduced in hy5 seeds.

A total of 43 lines were analysed by examining ABI5-HA protein level in individual transgenic plants (Fig. 1A). In the ABA-ABAR-WRKY40-ABI5 core signaling complex, WRKY40 acts to suppress ABA signaling, and high levels of ABA promote ABAR-WRKY40 interactions, then activate expression of ABA responsive genes, including the ABA responsive transcription factors ABI5 and ABI4. J. Exp. Bot., 2017.
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Bar, 1 cm. (D,E) Fresh weight and root length of abi5-1, ABI5-HA overexpression lines (OE) and WT seeds sown on plates containing DMSO, ABA (1 µM), and AZD (1 µM) for 10 days. Each graph 2016-12-20 Overexpression of ABI5 in plants to prevent precocious seed germination and to confer resistance to drought and high salt Mar 21, 2001 - The Rockefeller University The bZIP transcription factor ABI5 is shown to confer an enhanced response to exogenous abscisic acid during germination, seedling establishment and subsequent vegetative growth of plants. ABI5-mediated transactivation was inhibited by overexpression ofabi1-1, the dominant-negative allele of the protein phosphatase ABI1, and by 1-butanol, a competitive inhibitor of … 2008-03-10 SnRK1α1 Antagonizes Cell Death Induced by Transient Overexpression of Arabidopsis thaliana ABI5 Binding Protein 2 (AFP2) September 2020 Frontiers in Plant Science 11 Srinivas S. L. Gampala, Ruth R. Finkelstein, Samuel S. M. Sun, Christopher D. Rock 2019-08-09 Our data show that loss of FyPP function is sufficient to trigger ABI5-dependent inhibition of seed germination (Figure 2B), which is very similar to the previous report that overexpression of a SnRK2 kinase (PKABA1) in the ABI5-OE background is sufficient to activate ABI5 protein and suppress seed germination (Piskurewicz et al., 2008).

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Overexpression of ABI5 reduces, whereas loss of function in the abi5 mutant increases, Cd accumulation in Arabidopsis, indicating the involvement of ABI5 in modulating Cd uptake. To identify transcriptional regulators involved in ABI5-mediated Cd accumulation, we searched for ABI5-interacting proteins and identified the Cd-induced R2R3-MYB TF MYB49 . Overexpression of ABI5 altered flowering time. (A) The expression level of ABI5-HA fusion protein in individual transgenic lines of p35S::ABI5-HA. Overexpression of ABI3 and ABI5 simultaneously suppressed the ABA-insensitive phenotypes of the coi1-2 mutant and JAZ- accumulating (JAZ-DJas) plants. Together, our results reveal a previously uncharacterized signaling module in which JAZ Moreover, overexpression of ABI5 (ABI5-OE-8) in the JAZ8-ΔJas ABI3-OE background rendered the transgenic lines much more sensitive to ABA and COR during seed germination . Similar data were also obtained when ABI3 and ABI5 were expressed in the JAZ5-ΔJas background ( Supplemental Figures 12C and 12D ).